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宿主来源
Rabbit抗原名称
LRRK2分子别名
Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin; PARK8免疫原
Synthetic Peptide细胞定位
Mitochondrion, Cytoplasm, Endoplasmic reticulum, Cytoskeleton, Lysosome, EndosomeAccession
Q5S007克隆号
S-2521-13抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu
Phospho-LRRK2 (S1292) refers to the specific form of the Parkinson's disease-associated protein LRRK2 that is phosphorylated at its serine 1292 residue. LRRK2 is a multifunctional kinase, and mutations in it are among the most common genetic causes of familial Parkinson's disease. The S1292 site is located within the WD40 domain of LRRK2, and its phosphorylation status is dynamically regulated by upstream kinases (such as PKA) and phosphatases (such as PP1). Phosphorylation at this site can influence LRRK2's kinase activity, GTPase activity, interactions with other proteins (e.g., 14-3-3 proteins), subcellular localization (such as binding to microtubules and membrane structures), and neurotoxicity. Research indicates that S1292 phosphorylation levels may be altered in LRRK2 mutants (e.g., G2019S) and are associated with the pathological mechanisms of Parkinson's disease (such as neuronal dysfunction and vesicle trafficking abnormalities). Therefore, it is considered a potential therapeutic target and biomarker. Detection of phosphorylation at this site is typically performed using specific antibodies for Western Blot or immunohistochemical analysis.
免疫印迹
WB result of Phospho-LRRK2 (S1292) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-LRRK2 (S1292) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LRRK2 WT transfected 293F whole cell lysate 20 µg
Lane 2: LRRK2 mutant G2019S transfected 293F whole cell lysate 20 µg
Lane 3: LRRK2 mutant S1292A transfected 293F whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 286 kDa
Observed MW: 286 kDa
This blot was developed with high sensitivity substrate
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